Lab-Tacular Tools

Solve C1V1 = C2V2 for any missing value.

Molecular weight and per-element composition from a chemical formula, with an optional name lookup.

Solve for mass, molecular weight, volume, or molarity from the other three.

Calculates per-step concentration plus stock and diluent volumes.

Calculates the stock and diluent volume needed for a target concentration and volume.

Carries the standard stock and working concentrations for nine antibiotics and works out the volume to add for as many bottles of medium as you like.

Calculates nucleic acid concentration, yield, and the A260/A280 and A260/A230 purity ratios.

Volumes to take from each library and buffer to add, for equimolar, equal-volume and weighted pooling.

Paste a whole delivery list and get the buffer volume for every tube at once, with clipboard and Excel export.

Paste your measurements to get the mean, standard deviation, and CV (%).

Fits a linear regression for slope, intercept, and R², then back-calculates unknown concentrations.

Fits a four-parameter logistic (4PL) through your standards and reads each sample's concentration back off it.

Calculates amplification efficiency (%) and the per-cycle factor from a standard curve slope.

How much of each component to pipette for a batch of reactions, with water filling the rest.

Finds a buffer pH, or the amount of each form to weigh out for a target pH, with Henderson-Hasselbalch.

Turns a gel volume and target percentage into agarose, buffer and stain volumes, and points at the percentage that resolves the fragment size you need.

Percentage, gel count and thickness in; resolving and stacking gel recipes out, plus the gel percentage for your protein size.

Turns haemocytometer counts into cells/mL, total cells and viability.

Suspension and medium for every vessel, from your current density and viability — several vessel types in one plan, with edge wells and bulk preparation handled.

Converts between %(w/v) concentration and molarity.

Converts between molarity and mass concentration through the molecular weight, with no stray factor of 1000.

Converts between RCF and RPM using rotor radius.

Converts nucleic acid mass, amount and copy number from length and per-unit molecular weight.

Turns a length in bp or nt into a molecular weight in daltons, and into the mass of one picomole.

Turns an A280 reading into mg/mL using your extinction coefficient — which it will help you calculate rather than guess.

Converts between absorbance and transmittance, on the common logarithm a spectrophotometer actually reports.

Fill in any one of pH, hydrogen ion concentration, pOH or hydroxide, and the other three follow.

Converts a temperature between Celsius, Fahrenheit and kelvin.

Converts a pressure between Pa, kPa, bar, psi, atm and mmHg (Torr), saying which factors are definitions.

Converts an energy between J, kJ, cal, kcal and eV, and makes you say which calorie you mean.

Calculates fold changes against a control group from qPCR Ct values.

Turns band densities into fold changes, normalised to a loading control.

Draws an error bar chart from grouped data, with the summary statistics.

Works out what goes in a ligation reaction from the molar ratio you want.

Calculates transformation efficiency in cfu/µg from colony counts.

Works out how many subjects an experimental group needs for a given power.

Finds the growth rate and doubling time from a time course.

Fits a sigmoid to dose–response data and returns the half-maximal concentration and the Hill slope.

Km, Vmax and Vmax/Km from substrate concentrations and initial rates, with the fitted curve.

Picks the statistical test that fits your data, and runs it.

Paste your QC values for control limits and a Westgard six-rule verdict in plain sentences.

Test the most extreme value in a set of replicates and see the statistic, the critical value and the p value.